Table 1 Primer sequences
From: Poor maternal nutrition inhibits muscle development in ovine offspring
Gene | Primer sequences (5’ → 3’) | Amplicon length | Reference |
|---|---|---|---|
Pax7 | Â | Â | Â |
Forward | GAGACCGACTGCTGAAGGAC | 167 | XM_002685738 |
Reverse | ATGCTGTGCTTGGCTTTCTT | Â | Â |
Myf5 | Â | Â | Â |
Forward | AGACGCCTGAAGAAGGTGAA | 134 | XM_004006219.1 |
Reverse | AGCAGCTCCTGCAGACTCTC | Â | Â |
Myogenin | Â | Â | Â |
Forward | TGGGCGTGTAAGGTGTGTAA | 169 | Tong et al., 2009 [12] |
Reverse | TGCACAGGATCTCCACTTTG | Â | Â |
MyoD | Â | Â | Â |
Forward | CCCTGGTGACTTCAGCTGTT | 239 | Tong et al., 2009 [12] |
Reverse | CCTGCCTGCCGTATAAACAT | Â | Â |
Myostatin | Â | Â | Â |
Forward | CACAGAAGGTCTTCCCCTCA | 147 | NM_001001525.2 |
Reverse | GGTTAAATGCCAACCATTGC | Â | Â |
Follistatin | Â | Â | Â |
Forward | AAAACCTACCGCAACGAATG | 120 | NM_001257093.1 |
Reverse | GAGCTGCCTGGACAGAAAAC | Â | Â |
Foxo1 | Â | Â | Â |
Forward | TGACTTGGACGGCATGTTTA | 157 | XM_004012275.1 |
Reverse | CCAGCTGTGTGTTGTCGTCT | Â | Â |
Foxo3a | Â | Â | Â |
Forward | GGGGAGTTTGGTCAATCAGA | 170 | NM_001267889.1 |
Reverse | TTTGCATAGACTGGCTGACG | Â | Â |
GAPDH 1 | Â | Â | Â |
Forward | GGCGTGAACCACGAGAAGTATAA | 118 | Buza et al., 2009 [21] |
Reverse | CCCTCCACGATGCCAAAGT | Â | Â |