Figure 2

Poor maternal nutrition alters lipid accumulation in the semitendinosus muscle of offspring. Cross sections (10 μm) of the semitendinosus muscle were stained with wheat germ agluttinin (WGA; red) to delineate the muscle fiber membrane, BODIPY 493/503 (green) which stains lipids and Hoescht 33342 (blue) to identify nuclei. Representative photographs are shown (A). Data from 1 d (B) and 3 mo of age (C) are presented as mean ± SEM. **P < 0.0001 vs. CON; * P < 0.05 vs. CON. CON = lambs from control-fed ewes (100% NRC), OVER = lambs from overfed ewes (140% NRC), RES = lambs from restricted-fed ewes (60% NRC), n = 3 lambs/group.