Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase
© Meadus et al.; licensee BioMed Central Ltd. 2014
Received: 11 July 2013
Accepted: 24 December 2013
Published: 3 January 2014
Camelina sativa is an oil seed crop which can be grown on marginal lands. Camelina seed oil is rich in omega-3 fatty acids (>35%) and γ-tocopherol but is also high in erucic acid and glucosinolates. Camelina meal, is the by-product after the oil has been extracted. Camelina meal was fed to 28 d old weaned pigs at 3.7% and 7.4% until age 56 d. The camelina meal supplements in the soy based diets, improved feed efficiency but also significantly increased the liver weights. Gene expression analyses of the livers, using intra-species microarrays, identified increased expression of phase 1 and phase 2 drug metabolism enzymes. The porcine versions of the enzymes were confirmed by real time PCR. Cytochrome 8b1 (CYP8B1), aldehyde dehydrogenase 2 (Aldh2), and thiosulfate transferase (TST) were all significantly stimulated. Collectively, these genes implicate the camelina glucosinolate metabolite, methyl-sulfinyldecyl isothiocyanate, as the main xeniobiotic, causing increased hepatic metabolism and increased liver weight.
KeywordsCamelina meal Gene expression Glucosinolates Pig liver
Camelina sativa, a member of the family Brassicaceae is related to rapeseed . It has commercial value as an oil seed crop for biofuels and biolubricants and can be grown on marginal lands . Camelina seed has an oil content of over 40% (dry weight) and this oil is high in omega-3 fatty acids, gamma tocopherol  but also in the monounsaturated omega-9 fatty acid, erucic acid (C22:1 ω-9) . Typical seed crushers will extract the oil content from the seed down to 4% (dry weight) leaving the meal. The Camelina meal still has a problem because, after the oil has been extracted, it can have a total glucosinolate content of ~ 24 μmol/g . In canola meal, when total glucosinolate content is higher than 15 μmol/g of feed, it will reduce feed intake and growth in finishing pigs [6, 7].
Glucosinolates are considered bitter to humans . Chickens , fish (trout)  and pigs  will initially show reduced feed intakes depending on the dosage. The maximum recommended dose of glucosinolates for monogastric animals, such as swine, is approximately 2 umoles/g of feed. The glucosinolates are metabolized by endogenous plant enzymes called myrosinase or β-thioglucosidases from gut bacteria, into biologically active compounds, isothiocyanates, indoles and nitriles . Higher doses of the glucosinolate metabolite, thiocyanate, can affect the transport of iodine to thyroid . Glucosinolate metabolic products are mainly associated with the induction of Phase I and Phase 2 biotransformation enzymes . Phase 1 enzymes catalyse a variety of hydrolytic, oxidative and reductive reactions, including the cytochrome P450s involved in metabolizing xenobiotics and toxins . Phase 2 enzymes, such as glutathione S-transferase and UDP-glucuronyl transferase, form conjugation products with xenobiotics and are readily excreted . When feeding xenobiotics, liver is typically the most responsive tissue for phase 1 and 2 expression. However, the phase 1 and 2 enzymes are expressed almost ubiquitously throughout the body, in multiple species, including humans.
Glucosinoates metabolites from camelina, rapeseed and canola differ slightly in the type and quantity. Glucosinolates metabolites from rapeseed and Canadian canola (Brassica napsis) are predominately progoitrin (2-hydroxy-3-butenyl) and gluconapin (3-butenyl) structure . Glucosinolates from camelina are predominantly glucocamelina, which is metabolized into 10-methylsulphinyldecyl isothiocyanate . The structure of 10-methylsulfinyldecyl is closely analogous to sulforaphane which is common to cruciferous vegetables such as broccoli, mustard and cabbage and may be protective against cancer and cardiovascular disease . The present study was undertaken as a preliminary investigation of pigs receiving camelina meal as alternative feed source and as an animal model to assess the potential health benefits.
Animals and feed
Chemical analysis of camelina meal’s crude fat, crude protein and glucosinolate content
Composition of the experimental cornstarch soy-concentrate-based diets without (Control) or with Low (37 g/kg) or High (74 g/kg) levels of added camelina meal
Dicalcium phosphate (21%)
Magnesium oxide (56%)
Trace mineral mix
Choline chloride (60%)
Selenium (1,000 mg/kg)
ADE vitamin mix2
Vitamin E (1,000 IG/g)
Calculated contents, g/kg1
Dry matter (DM)
Total glucosinolates, μmol/kg
Daily feed intake, average daily gain, feed conversion efficiency and organ tissue weights of pigs after a 28 d trial of cornstarch soy-concentrate-based diets without (Control) or with Low (3.7 g/kg) or High (7.4 g/kg) levels of added camelina meal
Start weight (kg)
Final weight (kg)
Average daily gain (g/d)
Average feed intake (g/d)
Feed conversion (feed/gain)
Organ tissue, g
RNA microarray analysis and quantitative PCR
Total pig liver RNA was examined for gene expression changes by microarray analysis using, the Rat Drug metabolism: phase 1 array (PARN-068) and the Human Drug Metabolism: phase 2 array (PAHS-069) (SABioscience /Qiagen, Mississauga, ON). Briefly, Liver samples were collected from the pigs within 10 min post slaughter and stored at -80°C in RNAlater (Qiagen). Total RNA was extracted from the livers (100 mg) by homogenizing in 5 moles/L guanidium isothiocyanate and then binding on silica columns for DNase treatment and washing before collecting the RNA in water according to the manufacturer’s methods of the Aurum Total RNA fatty and fibrous tissue kit (BioRad, Mississauga, Ontario). The quality and quantity of the RNA was assessed spectrophotometrically at 260 nm, 280 nm and 320 nm. The RNA quality was also checked for good, intact, 28S and 18S rRNA bands by visualization on a 1.0% agarose gel electrophoresis stained with 1 ug/mL ethidium bromide
For microarray analysis, total RNA was collected from the control fed pigs and the 7.4% camelina fed pigs and pooled within the three treatments and was converted to cDNA with a RT2 First Strand Kit (Qiagen Canada, Mississauga, ON). The pooled cDNA was tested by microarray analysis, on the Rat drug metabolism: phase 1 array (PARN-068) and the Human Drug Metabolism: phase 2 array (PAHS-069) (SABioscience /Qiagen, Mississauga, ON) using the RT2 SYBR Green polymerase chain reaction (PCR) Master mix according to manufacturer’s recommendations on a Mx3000P QPCR real time PCR machine with SYBR gene detection (Agilent Technologies Canada Inc., Mississauga, ON). PCR conditions were hot start at 95°C for 10 min followed by 95°C for 15 sec, 55°C for 15 s, and 60°C for 60 s, for 40 cycles. Quantitative gene analysis was based on comparative 2-ΔΔCt method using the RT2 profiler PCR data analysis program 3.5. (SABiosciences/ Qiagen, Mississauga, ON).
The genes identified by the arrays were used in the search for their closest porcine equivalent in the National Center for Biotechnology Information (NCBI) U.S. National Library of Medicine, GenBank  using the Basic Local Alignment Search Tool (Blast) version 2.2.27 program. Primers sequences were selected, using the Primer 3 program v.0.4.0, to amplify the porcine version of the gene transcript . Porcine transcripts were confirmed by sequencing the PCR products on a CEQ8000 machine using a GenomeLab™ DTCS Quick Start dideoxy sequencing kit as per manufacturer’s instructions (Beckman Coulter, Mississauga, ON, Canada).
Forward and reverse porcine primer sequences and accession numbers of genes used in quantitative RT-PCR analysis
GenBank accession #
cDNA size, bp
Animals’ responses to the diets were analyzed using ANOVA followed by Duncan’s test for differences in the group means. The gene analysis data was calculated using the comparative 2-ΔΔCt method and significance between treatment groups was determined using the Student’s t-test. Statistical significance was accepted at P < 0.05 and trends were indicated at P < 0.1. All data were run on SAS version 9 .
Results and discussion
The estimated chemical composition of the camelina meal supplemented diets for crude protein and crude fat are outlined (Tables 1 and 2). The amount of crude protein in the meal was estimated to be ~363 g/kg and the amount of crude fat was 143 g/kg, which is high at ~14%, considering the oil was extracted by cold pressure . The concentration of glucosinolates was measured to be ~23.70 μmol/g in the camelina meal using a chromatography method . This content is significantly higher than the average value of 7.8 μmol glucosinolate /g of meal from modern conventional varieties of canola . However, the estimated final concentration of glucocamelinin in the LOW and HIGH supplemented diets was well within the safe region at 0.82 µmol/kg and 1.63 μmol/kg of feed (Table 2).
Camelina contains a high concentration of the antioxidant, gamma tocopherol 1,000 μg/g, which is substantially higher, relative to other oils seed crops such as, canola and flax . Gamma tocopherol is the most commonly found form of vitamin E in plants . It provides antioxidant protection to the oils and gives a nutty aroma to camelina oil. For humans however, alpha-tocopherol is the preferred form and the only isomer selected by the tocopherol transfer protein (α-TTP) and tocopherol associated protein (α-TAP) in the liver . Gamma tocopherol is absorbed but quickly excreted in the urine.
In addition to glucosinolates, Camelina also contains the monounsaturated omega-9 fatty acid, erucic acid. Erucic acid (C20:1 w-9) is a large component of mustard oil and can account to approximately 5% of camelina seed and is suspected to reduce the palatability of feed for monogastric swine . The allowable amount of erucic acid in canola seed in Canada is set at 2% . Erucic acid content is a large component of mustard oil and its consumption by human is still controversial. It implicated in causing thrombocytopenia but it is also part of Lorenzo’s oil  used to the treat adrenoleukodystrophy . The crude fat in meal of camelina after crushing and processing was 14.3% and this fat is expected to contain less than 0.715 g erucic acid/kg of meal. The level of erucic acid in the LOW diet was expected to be 0.026 g/kg feed. In the HIGH diet, the erucic acid content was estimated to be 0.053 g/kg of feed (~ 0.005%), which is below the level expected for any detectable physiological effect .
Animals and weights
Mean initial weights of the pigs were 12.7 ±1.73 kg for the start of trial and at the end of the 28 day trial; the pigs weighed an average of 17.1 ±2.12 kg at 56 d (Table 3). The animals on the camelina meal supplemented diets had a significant improvement in their feed efficiency. This may have been due to an increase in the available lysine protein and metabolizable energy available from the camelina meal supplement; although, the soy concentrate, corn starch based control diet, met NRC  recommended lysine levels estimated at ~ 14.6 g/kg feed for 20 kg pigs (Table 2). It may have been also due to a reduced feed intake in the LOW and HIGH diets (Table 3). The pigs did have some problems adjusting to the camelina meal diets. The pigs on HIGH diet did have an initial aversion to the meal, with reduced average daily feed intakes of 518.1 ±70.4 g/d, as compared to the CON diet intake at 605.9 ±111.3 g/d but this was only shown as a trend P < 0.1. There was a significant increase in the liver weights from the pigs fed the HIGH diet. The liver weights at the end of the trial was 346.4 g/pig for the CON group, 418.4 g/pig for MED group and 427.7 g/pig for HIGH fed group (Table 3). This indicates extra hepatic activity caused by increasing the camelina meal. The pigs fed the LOW and HIGH camelina meal diet also indicated a reduction in their thyroid weights (Table 3). This may be due to a disruption of iodine absorption and thyroid activity caused by glucoinosinolates in the camelina meal and will have to investigated further; possibly in a longer (>28 d) trial in which the iodine level in the pigs are measured.
Hepatic gene expression
The rat Drug Metabolism Phase 1 microarray identified the cytochrome P450 -8b1 (Cyp8b1) and the aldehyde dehydrogenase 2 (Aldh2) gene transcription as being stimulated in the pig livers by camelina meal feeding. The human drug metabolism phase 2 array identified glutathione S-transferase mu 5 (GSTM5) and thiosulfate transferase (TST), as being significantly up-regulated > 4-fold (Figure 1) relative to the control livers, as determined by the RT2 profiler PCR data analysis program 3.5.
A comparison of the gene sequences identified in the rat array and porcine (Sus scrofa) version of the genes was 87% between the rat Aldh2 GenBank# NM_032416 and the porcine GenBank# NM_001044611.2 and 80% between the rat Cyp8b1 GenBank# NM_031241 and the porcine GenBank# NM_214426.1. Comparison of the genes identified in the human array with porcine (Sus scrofa) mRNA was 86% between the human GSTm5 and the porcine GSTm2 GenBank# AK233626.1 and 89% between the human TST GenBank# NM_003312 and the porcine TST GenBank# XM001926303.2. New porcine specific primers were created based on the aforementioned porcine sequences (Table 4).
Cytochrome P450 8B1 (Cyp8b1) is primary a microsomal sterol hydroxylase involved in bile acid formation . Porcine Cyp8b1 will catalyse the hydroxylation of cholic acid into hyocholic acid. This activity is developmentally dependent giving higher expression in the fetal than the adult pig liver. The basic structure of cholic acid is cholesterol, which is quite different from the metabolites of camelina meal. Glucosinolates of camelina are mainly glucocamelina, which is a methyl-sulfinyldecyl isothiocyanates . The transcriptional activation Cyp8b1 is probably the same mechanism as caused by broccoli. Metabolites of broccoli glucosinolate contain sulforaphane which is a methyl-sulfinylbutane isothiocyanate. Sulforaphane activates phase 2 drug enzymes by the nuclear factor, E2 p-45-related factor 2 (Nrf2) transcription factor . The Nrf2 factor binds the antioxidant response element (ARE) to activate transcription of the respective genes . The same Nrf2 transcription factors have been shown to activate the other phase 2 enzymes induced by camelina meal including the Aldh2, TST and GstM1 . Future trials should examine if camelina thiocyanate and its derivatives are actually inducing the Nrf2 transcription factor and the genes which respond to the ARE, such as the phase 1 enzyme Cyp1A1.
Camelina sativa has high erucic acid and high glucosinolate content but it can be grown on marginal land and has good oil yield. The continued industrial use of camelina oil as a biofuel may provide a cheap source of protein as meal for the pig industry but more study into the nutritional aspects is needed. Recent information on the biological action of glucosinolates and erucic acid has suggested that these are not as detrimental as originally thought . Rats are relatively slow at digesting erucic acid and due to its accumulation in the heart , thought to be cardiotoxic  but recent studies have shown that the animals will adjust to the feed and increase erucic acid metabolism within their peroxisomes . Glucoinsolates were thought to be only anti-nutritional but now the research evidence has demonstrated that glucosinolates may act as a chemo-protective, anti-cancer agent . This trial on camelina meal feeding in pigs, indicates a potential anti-carcinogen benefit, through the stimulation hepatic expression of phase 1 and 2 xenobiotic detoxifying enzymes.
Cytochrome P450 8b1
GlutathioneS- transferase mu 5
Polymerase chain reaction
This work was supported by Agriculture and Agri-food Canada. The authors would also like to thank the staff as the Lacombe piggery for their assistance.
- Gehringer A, Friedt W, Luhs W, Snowdon RJ: Genetic mapping of agronomic traits in false flax (Camelina sativa subsp. sativa). Genome. 2006, 49: 1555-1563. 10.1139/g06-117.View ArticlePubMed
- Gesch RW, Archer DW: Double-cropping with winter camelina in the northern Corn Belt to produce fuel and food. Ind Crop Prod. 2013, 44: 718-725.View Article
- Zubr J, Matthaus B: Effects of growth conditions on fatty acids and tocopherols in < i > Camelina sativa</i > oil. Industrial crops and products. 2002, 15: 155-162. 10.1016/S0926-6690(01)00106-6.View Article
- Ni Eidhin D, Burke J, Lynch B, O’Beirne D: Effects of dietary supplementation with camelina oil on porcine blood lipids. J Food Sci. 2003, 68: 671-679. 10.1111/j.1365-2621.2003.tb05730.x.View Article
- Schuster A, Friedt W: Glucosinolate content and composition as parameters of quality of < i > Camelina</i > seed. Industrial crops and products. 1998, 7: 297-302. 10.1016/S0926-6690(97)00061-7.View Article
- Bell JM: Nutrients and toxicants in rapeseed meal: a review. J Anim Sci. 1984, 58: 996-1010.PubMed
- Caine WR, Aalhus JL, Dugan MER, Lien KA, Larsen IL, Costello F, McAllister TA, Stanford K, Sharma R: Growth performance, carcass characteristics and pork quality of pigs fed diets containing meal from conventional or glyphosate-tolerant canola. Can J Anim Sci. 2007, 87: 517-526. 10.4141/CJAS07028.View Article
- Mithen RF, Dekker M, Verkerk R, Rabot S, Johnson IT: The nutritional significance, biosynthesis and bioavailability of glucosinolates in human foods. J Sci Food Agric. 2000, 80: 967-984. 10.1002/(SICI)1097-0010(20000515)80:7<967::AID-JSFA597>3.0.CO;2-V.View Article
- Pekel AY, Patterson PH, Hulet RM, Acar N, Cravener TL, Dowler DB, Hunter JM: Dietary camelina meal versus flaxseed with and without supplemental copper for broiler chickens: live performance and processing yield. Poult Sci. 2009, 88: 2392-2398. 10.3382/ps.2009-00051.View ArticlePubMed
- Morais S, Edvardsen RB, Tocher DR, Bell JG: Transcriptomic analyses of intestinal gene expression of juvenile Atlantic cod (Gadus morhua) fed diets with Camelina oil as replacement for fish oil. Comp Biochem Physiol B Biochem Mol Biol. 2012, 161: 283-293. 10.1016/j.cbpb.2011.12.004.View ArticlePubMed
- Schone F, Jahreis G, Lange R, Seffner W, Groppel B, Hennig A, Ludke H: Effect of varying glucosinolate and iodine intake via rapeseed meal diets on serum thyroid hormone level and total iodine in the thyroid in growing pigs. Endocrinologia experimentalis. 1990, 24: 415-427.PubMed
- Zhang Y, Talalay P, Cho CG, Posner GH: A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proc Natl Acad Sci U S A. 1992, 89: 2399-2403. 10.1073/pnas.89.6.2399.PubMed CentralView ArticlePubMed
- Gross-Steinmeyer K, Stapleton PL, Tracy JH, Bammler TK, Strom SC, Eaton DL: Sulforaphane- and phenethyl isothiocyanate-induced inhibition of aflatoxin B1-mediated genotoxicity in human hepatocytes: role of GSTM1 genotype and CYP3A4 gene expression. Toxicol Sci. 2010, 116: 422-432. 10.1093/toxsci/kfq135.PubMed CentralView ArticlePubMed
- Nho CW, Jeffery E: The synergistic upregulation of phase II detoxification enzymes by glucosinolate breakdown products in cruciferous vegetables. Toxicol Appl Pharmacol. 2001, 174: 146-152. 10.1006/taap.2001.9207.View ArticlePubMed
- Shahidi F, Gabon JE: Individual glucosinolates in six canola varieties. J Food Qual. 1989, 11: 421-431. 10.1111/j.1745-4557.1989.tb00905.x.View Article
- Kjaer AG R, Jensen RB: Isothiocyanates XXI. 10-methylsuphinyldecyl isothiocyanate, a new mustard oil present as a glucoside (glucocamelinin) in Camelina species. Acta Chem Scand. 1956, 10: 1614-1619.View Article
- Anwar-Mohamed A, El-Kadi AO: Sulforaphane induces CYP1A1 mRNA, protein, and catalytic activity levels via an AhR-dependent pathway in murine hepatoma Hepa 1c1c7 and human HepG2 cells. Cancer Lett. 2009, 275: 93-101. 10.1016/j.canlet.2008.10.003.View ArticlePubMed
- NRC: Nutrient Requiements of Swine. 1998, Washington DC: National Academy of Sciences, 10
- CCAC: CCAC Guidelines on: The Care and Use of Farm Animals in Research, Teaching, and Testing. 2009, Ottawa, ON: Canadian Council on Animal Care, 8
- Benson DA, Karsch-Mizrachi I, Clark K, Lipman DJ, Ostell J, Sayers EW: GenBank. Nucleic Acids Res. 2012, 40: D48-D53. 10.1093/nar/gkr1202.PubMed CentralView ArticlePubMed
- Primer3 on the WWW for General Users and for Biologist Programmers. Edited by: Rozen SHJS. 2000, Totowa, NJ, USA: Humana Press
- Meadus WJ, Duff P, Rolland D, Aalhus JL, Uttaro B, Dugan MER: Feeding docosahexaenoic acid to pigs reduces blood triglycerides and induces gene expression for fat oxidation. Can J Anim Sci. 2011, 91: 601-612. 10.4141/cjas2011-055.View Article
- Schmittgen TD, Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nature protocols. 2008, 3: 1101-1108. 10.1038/nprot.2008.73.View ArticlePubMed
- SAS user’s guide: Stastics. SAS for Windows, Version 9.1. Edited by: SAS. 2003, Cary, NC, USA: SAS Institute Inc
- AOCS/Ak-1-92: Official methods and recommended practices of the American Oil Chemists’ Society 6th ed 2nd print Champain, Ill. Determination of Glucosinolate Content in Rapeseed and Canola by HPLC method Ak 1-92. 2012, 8.
- Wagner KH, Kamal-Eldin A, Elmadfa I: Gamma-tocopherol–an underestimated vitamin?. Ann Nutr Metab. 2004, 48: 169-188. 10.1159/000079555.View ArticlePubMed
- Doring F, Rimbach G, Lodge JK: In silico search for single nucleotide polymorphisms in genes important in vitamin E homeostasis. IUBMB life. 2004, 56: 615-620. 10.1080/15216540400020346.View ArticlePubMed
- Habeanu M, Hebean V, Taranu I, Ropota M, Lefter N, Marin D: Dietary ecologic camelina oil - a beneficial source of n-3 PUFA in muscle tissue and health status in finishing pigs. Romanian Biotechnological Letters. 2011, 16: 6564-6571.
- CFIA-DD96-07: Canadian Food Inspection Agency, vol. DD96-07 supplement. Determination of Environmental Safety of Monsanto Canada Inc.’s Roundup® Herbicide-Tolerant Brassica napus Canola Line GT200. 2011
- Borg K: Physiopathological effects of rapeseed oil: a review. Acta Med Scand Suppl. 1975, 585: 5-13.PubMed
- Lundell K, Wikvall K: Gene structure of pig sterol 12alpha-hydroxylase (CYP8B1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6alpha-hydroxylase (CYP4A21). Biochimica et biophysica acta. 2003, 1634: 86-96. 10.1016/j.bbalip.2003.09.002.View ArticlePubMed
- Thimmulappa RK, Mai KH, Srisuma S, Kensler TW, Yamamoto M, Biswal S: Identification of Nrf2-regulated genes induced by the chemopreventive agent sulforaphane by oligonucleotide microarray. Cancer Res. 2002, 62: 5196-5203.PubMed
- Huang J, Tabbi-Anneni I, Gunda V, Wang L: Transcription factor Nrf2 regulates SHP and lipogenic gene expression in hepatic lipid metabolism. Am J Physiol Gastrointest Liver Physiol. 2010, 299: G1211-G1221. 10.1152/ajpgi.00322.2010.PubMed CentralView ArticlePubMed
- Qin S, Chen J, Tanigawa S, Hou DX: Microarray and pathway analysis highlight Nrf2/ARE-mediated expression profiling by polyphenolic myricetin. Mol Nutr Food Res. 2013, 57: 435-446. 10.1002/mnfr.201200563.View ArticlePubMed
- Murphy CC, Murphy EJ, Golovko MY: Erucic acid is differentially taken up and metabolized in rat liver and heart. Lipids. 2008, 43: 391-400. 10.1007/s11745-008-3168-3.View ArticlePubMed
- de Wildt DJ, Speijers GJ: Influence of dietary rapeseed oil and erucic acid upon myocardial performance and hemodynamics in rats. Toxicol Appl Pharmacol. 1984, 74: 99-108. 10.1016/0041-008X(84)90275-8.View ArticlePubMed
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