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Table 1 Cell culture media 1

From: Culture conditions for equine bone marrow mesenchymal stem cells and expression of key transcription factors during their differentiation into osteoblasts

Media type

Composition of media

Maintenance media

α-MEM + 10% FBS + 1% PS + 1% AB

Proliferation media

α-MEM + 0.1% BSA + 1% PS + 1% AB

ALP media

α-MEM + 0.1% BSA + 1% PS + 1% AB + VC + BG

Differentiation media

α-MEM + 10% FBS + 1% PS + 1% AB + VC + BG + DEX

  1. 1ALP = alkaline phosphatase; AB = amphotericin B; α-MEM = α-minimum essential medium; BSA = bovine serum albumin; BG = glycerol 2-phosphate disodium salt hydrate; DEX = dexamethasone; FBS = fetal bovine serum; PS = penicillin/streptomycin concentrate; VC = L-ascorbic acid 2-phosphate sesquimagnesium salt.
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891