Figure 2

The expression and subcellular localization of PAK1^Thr423and histone H3^Ser10during porcine oocyte meiotic maturation. (A) Representative images of PAK1^Thr423 (red), H3^Ser10 (green) and DAPI (blue) immunostaining in GV, GVBD, MI and MII oocytes. (B) A representative western blot showing the expression level of PAK1^Thr423 and H3^Ser10 at the GV, GVBD, MI and MII stages of oocyte maturation. (C) Representative images of chromosomal spreads showing MI oocytes costained with H3^Ser10 (green), PAK1^Thr423 (red) and DAPI (blue). Bar = 10 μm.