Low crude protein formulation with supplemental amino acids for its impacts on intestinal health and growth performance of growing-finishing pigs

Table 4 Primers used for real-time PCR

Gene	Primer	Sequence (5'→3')	Size, bp	Accession number
ASCT2	Forward	GCCAGCAAGATTGTGGAGAT	206	DQ231578
ASCT2	Reverse	GAGCTGGATGAGGTTCCAAA
B⁰AT1	Forward	CACAACAACTGCGAGAAGGA	155	DQ231579
B⁰AT1	Reverse	CCGTTGATAAGCGTCAGGAT
CAT-1	Forward	TGCCCATACTTCCCGTCC	192	NM_001012613
CAT-1	Reverse	GGTCCAGGTTACCGTCAG
b^0,+AT	Forward	ATCGGTCTGGCGTTTTAT	144	NM_001110171
b^0,+AT	Reverse	GGATGTAGCACCCTGTCA
y⁺LAT1	Forward	GCCCATTGTCACCATCATC	216	NM_001110421
y⁺LAT1	Reverse	GAGCCCACAAAGAAAAGC
4F2hc	Forward	CTCGAACCCACCAAGGAC	174	XM_003361818
4F2hc	Reverse	GAGGTGAGACGGCACAGAG
Pept-1	Forward	CCCAGGCTTGCTACCCAC	144	NM_214347
Pept-1	Reverse	ACCCGATGCACTTGACGA
rBAT	Forward	TTTCCGCAATCCTGATGTTC	146	NM_001123042
rBAT	Reverse	GGGTCTTATTCACTTGGGTC
β-actin	Forward	TGCGGGACATCAAGGAGAAG	216	XM_003357928
β-actin	Reverse	AGTTGAAGGTGGTCTCGTGG

ISSN: 2049-1891