Hesperidin ameliorates H2O2-induced bovine mammary epithelial cell oxidative stress via the Nrf2 signaling pathway

Table 1 The sequences of primers used for RT‒qPCR

Gene	Primer sequences (5'→3')	Product length, bp
HO-1	F: GAACGCAACAAGGAGAAC R: CTGGAGTCGCTGAACATAG	162
Nrf2	F: CCTCAAAGCACCGTCCTCAG R: GCTCATGCTCCTTCTGTCGT	168
NQO1	F: AACCAACAGACCAGCCAATC R: TCTATGGCAGCCTCCTTCAT	154
Keap1	F: CTGTCCTCAACCGTCTGCTC R: ATCCGCCACTCGTTTCTCTC	100
p38 MAPK	F: CGCCTGGCATATGTTTCTGAC R: CTCTGACACCCAAGTGGAGAC	175
GAPDH	F: GGCGTGAACCACGAGAAGTATAA R: CCCTCCACGATGCCAAAGT	120

F Forward, R Reverse, HO-1 Heme oxygenase-1, Nrf2 Nuclear factor erythroid 2-related factor 2, NQO1 NAD(P)H quinone oxidoreductase 1, Keap1 Kelch-like ECH-associated protein 1, p38 MAPK p38 mitogen-activated protein kinase, GAPDH Glyceraldehyde-3-phosphate dehydrogenase

ISSN: 2049-1891